Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
نویسندگان
چکیده
منابع مشابه
Development of a quantitative recombinase polymerase amplification assay with an internal positive control.
It was recently demonstrated that recombinase polymerase amplification (RPA), an isothermal amplification platform for pathogen detection, may be used to quantify DNA sample concentration using a standard curve. In this manuscript, a detailed protocol for developing and implementing a real-time quantitative recombinase polymerase amplification assay (qRPA assay) is provided. Using HIV-1 DNA qua...
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Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard a...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2015
ISSN: 1940-087X
DOI: 10.3791/52620